MLT Intact Proinsulin Luminescence Assay

The Invitron Intact Proinsulin Assay is an immunometric assay for the quantitative measurement of intact proinsulin in human samples.

Key features include:

Easy to use – very simple assay protocol
High sensitivity detection system
High specificity for ‘intact’ proinsulin
Highly specific marker for insulin resistance in type 2 diabetes
Calibrated against WHO International Standard (IRP 09/296)

Also available:

Intact Proinsulin ELISA

Total Proinsulin luminescence assay

Characteristics

Format	96 well plate
Specimen	plasma (recommended) or serum
Sample volume	50μl
Working range	0 – 100 pmol/l
Sensitivity	0.02 pmol/l
Specificity	Proinsulin 100% Insulin 0% C-Peptide 0% 32-33 split proinsulin: 5.6% Des 31-32 split proins: 1.4% 65-66 split proinsulin: 37%* Des 64-65 split proins: 63%* *not present at detectable levels in human samples
Incubations	1 x 2 hours, 1 x 1 hour
Product code	IV2-002

Protocol

Add 50 μl sample buffer and 50 μl sample to each well

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Incubate for 2 hours at 37°C

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Wash 3 times and add 100 μl labelled antibody solution

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Incubate for 1 hour at 37°C

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Wash 3 times

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Read in luminometer

Cross reactivity table.pdf

Product information sheet.pdf

Sample Publications

Insulin and proinsulin measurements in T2 diabetes.pdf