Total Proinsulin Luminescence Assay Kit

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MLT Total Proinsulin Luminescence Assay

The Invitron Total Proinsulin Assay is an immunometric assay for the quantitative measurement of total proinsulin in human samples.

Key features include:

  • Easy to use – very simple assay protocol

  • High sensitivity detection system

  • Calibrated against WHO International Standard (IRP 09/296)

Characteristics

Format

96 well plate

Specimen

serum or plasma

Sample volume

25μl

Working range

0 – 250mU/l

Sensitivity

0.25 mU/l

Specificity

Proinsulin 100%

Insulin 2.2%

C-Peptide 0%

32-33 split proinsulin: 97%

Des 31-32 split proins: 100%

65-66 split proinsulin: 12%*

Des 64-65 split proins: 1%*

*not present at detectable

levels in human samples

Incubations

1 x 2 hours, 1 x 1 hours

Product code

IV2-003

Cross reactivity table

Sample Publications

Product information sheet

Protocol

Add 50 μl sample buffer and 50 μl sample to each well

↓

Incubate for 2 hours at 37°C

↓

Wash 3 times and add 100 μl labelled antibody solution

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Incubate for 1 hour at 37°C

↓

Wash 3 times

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Read in luminometer