ox-LDL ELISA

The Invitron ox-LDL ELISA is an immunometric assay for the quantitative measurement of oxidised low-density lipoprotein (ox-LDL) in human samples.

Key features of the assay include:

  • Recognises MDA-modified apolipoprotein B 100.

Characteristics

Format

96 well plate

Specimen

serum or EDTA plasma

Sample volume

10μl

Working range

0 – 250 ng/ml

Incubations

2 x 1 hours, 1 x 15 min

Product code

IV3-105E

Protocol

Add 100 μl of diluted sample to each well

Incubate for 1 hour at room temp

Wash 5 times and add conjugate

Incubate for 1 hour at room temp

Wash 5 times and add substrate

Incubate for 15 min at room temp

Add stop solution and read at 450nm