Insulin ELISA

The Invitron Insulin ELISA is an immunometric assay for the quantitative measurement of insulin in human samples.

Key features of the assay include:

  • High specificity
  • Therapeutic insulin analogues can be measured using this assay
  • Calibrated against WHO International Standard (IRP 66/304)
Invitron ELISA kit

Characteristics

Format

96 well plate

Specimen

serum or plasma

Sample volume

25μl

Working range

0 – 250mU/l

Sensitivity

mU/l

Specificity

Insulin 100%

Proinsulin 1.2%

C-Peptide 0%

Incubations

1 x 2 hours, 1 x 30 min

Product code

IV2-101E

Protocol

Add 100 μl enzyme conjugate and 25 μl sample to each well

Incubate for 2 hours at 37°C

Wash 3 times and add substrate

Incubate for 15 min at room temp

Add stop solution and read at 450nm